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. 2022 Jan 25;17(1):e0262854. doi: 10.1371/journal.pone.0262854

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© 2022 Nakanishi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) CS disaccharide composition analysis. Representative chromatogram of the standard (left panel), the digestion of SCE-UCBCs with Chase ACII (UCBC-1, middle panel), and that with Chase ABC (UCBC-2, right panel). ΔDi-0S, deoxy-α-L-threo-hex-4-enopyranosyluronic acid (ΔUA) (1→3) N-acetylgalactosamine (GalNAc); ΔDi-4S, ΔUA(1→3)GalNAc4S, where S is sulfo; ΔDi-6S, ΔUA (1→3) GalNAc6S; ΔDi-UA2S, ΔUA2S (1→3) GalNAc; ΔDi-di4,6S, ΔUA (1→3) GalNAc4S6S; ΔDi-di2,4S, ΔUA2S (1→3) GalNAc4S; ΔDi-di2,6S, ΔUA2S (1→3) GalNAc6S; ΔDi-triS, ΔUA2S (1→3) GalNAc4S6S. (B) HS disaccharide composition analysis. Representative chromatogram of the standard (left panel) and digestion of SCE-UCBCs with heparinases (UCBC-1, right panel). ΔDi-0S, ΔUA (1→4) N-acetylglucosamine (GlcNAc); ΔDi-NS, ΔUA (1→4) N-sulfated glucosamine (GlcNS); ΔDi-6S, ΔUA (1→4) GlcNAc6S; ΔDi-NS6S, ΔUA (1→4) GlcNS6S; ΔDi-NSUA2S, ΔUA2S (1→4) GlcNS; ΔDi-TriS, ΔUA2S (1→4) GlcNS6S.