Fig 3. No alteration of protein anabolisms in cultured human skeletal muscle cells with hibernating bear serum.

(A) Representative images of Western blotting with antipuromycin antibody. Equal amounts of protein loading in each sample were confirmed by the staining with Coomassie Brilliant Blue. (B) Protein synthesis in cultured myotubes was determined by quantification (normalized to summer-active group) of the incorporated puromycin. The Animal ID indicates the identification number of each individual bear. The experimental group for each season was consisted of identical bear individuals. N = 7 in each group. Data are expressed as mean ± standard errors.