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. 2022 Jan 25;17(1):e0263085. doi: 10.1371/journal.pone.0263085

Fig 8. Gene expression levels of mitochondrial regulatory factors in cultured human skeletal muscle cells following hibernating bear serum treatment.

Fig 8

(A) Gene expressions of mitochondrial regulatory factors were determined (A-F). (A) pgc1α. (B) pgc1β. (C) ucp3. (D) cycs. (E) cox4. (F) cpt1β. Expression levels of each studied gene were determined by the 2–ΔΔCT method while referencing ACTB as an internal control. Absolute Ct values of ACTB PCR amplification for each sample were stable (between 15 to 16 cycles). The experimental group for each season was consisted of identical bear individuals. N = 7 in each group. Data are expressed as mean ± standard errors. Significant differences: #, p < 0.05.