Table 2. LAMP assays for detection of SARS-CoV-2 RNA on money cards on Brigham Young University (BYU) campus.
Date collected | COVID cases per 100,000 (daily)* | # assayed | # LAMP positive (%)** | # Plaque assay positive (%) |
---|---|---|---|---|
11/17/2020 | 122.3 (79.4) | 110 | 8 (7.2%) | - |
12/11/2020 | 92.1 (99.8) | 90 | 3 (3.3%) | - |
2/19/2021 | 29.4 (22.8) | 79 | 6 (7.6%) | 0*** |
Totals: | 279 | 17 (2.6%) |
*Active, laboratory confirmed, 7-day average COVID cases from Utah County as declared from the local health department (daily rate in parenthesis).
**Positive samples are positives from an initial assay that were verified in a secondary assay. LAMP assays were conducted using the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs).
***A total of 14 samples, including all 6 positive samples and 8 negatives from the 2/19/2021 testing were assayed in the BSL-3 laboratory for live virus using a dilution series starting at 1:4. No plaques were seen for any of the samples. A viable suspension of SARS-CoV-2 with a titer of 7.75x106 pfu/ml was used as a positive control. “-”is not determined.