Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 21;219(2):e20211295. doi: 10.1084/jem.20211295

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Nicolas et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S1. — Expression of CD4, CD90.1, CD3, and CD28 and TCR Vβ usage in SLP-76^OST–edited and control CD4⁺ T cells. (A) After gating on live singlets, SLP-76^OST–edited and control CD4⁺ T cells that were expanded for 34 d were analyzed by flow cytometry for the expression of CD4, CD90.1, CD3, and CD28. Numbers in dot plots and histograms indicate the percentage of cells within the specified gates and the percentage of positive cells, respectively. Data are representative of three independent experiments. (B) SLP-76^OST–edited and control CD4⁺ T cells were expanded for AP-MS and analyzed using a panel of 24 TCR Vβ–specific antibodies. The clonogram shows that both populations have preserved a rather similar polyclonal Vβ repertoire. Data are representative of two independent experiments. FSC, forward scatter; SSC, side scatter.