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. 2022 Jan 21;219(2):e20211295. doi: 10.1084/jem.20211295

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© 2022 Nicolas et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — Phosphorylation dynamics of the threonine residue found at position 341 of SLP-76. (A) Plot showing the log₁₀-transformed intensity for the phospho-site corresponding to the threonine residue found at position 341 of SLP-76 (SLP-76-pT341) over the course of TCR stimulation. Phosphorylated peptide intensity was quantified using AP-MS samples of SLP-76^OST human CD4⁺ T cells. Data corresponding to each of the three biological replicates are specified by dots of a distinct color. Norm., normalized. (B) Plot showing the correlation between SLP-76-pT341 intensity and the stoichiometry of the interaction between SLP-76 and its high-confidence prey 14-3-3ζ, both quantified from AP-MS data. Our dataset did not permit identification of additional phosphorylated peptides with high confidence in the SLP-76 bait and the other baits.