FIG 1.

Overview of the methodology used to determine enrichment of APOBEC-derived AA changes. To directly compare which AA changes occurred more frequently in mBC than in pBC, the catalog of all possible APOBEC-induced codon changes was replaced by observed APOBEC-induced codon changes in pBC. AA, amino acid; ALT, alternative base; APOBEC, Apolipoprotein B mRNA-Editing Catalytic Polypeptide-like; C, cysteine; E, glutamate; F, phenylalanine; G, glycine; K, lysine; L, leucine; mBC, metastatic breast cancer; N, asparagine; pBC, primary breast cancer; Q, glutamine; REF, reference base; S, serine; SBS, single base-pair substitution; T, threonine; V, valine; X, STOP (stop codon).