Fig. 3.

Effects of expression of either or both of the N. lactamica farA and farB genes on growth of the Aas-deficient Synechocystis sp. PCC 6803 mutant in the presence of 18:3. Three microliters of the Synechocystis cell suspensions (OD₇₃₀ = 1.0) and their 5-fold serial dilutions were spotted onto solid media and incubated for 5 d at 30°C under illumination at 25 µmol photons m⁻² s⁻¹. Growth of the cells in the absence (A) and the presence (B) of 0.1 mM 18:3 is compared. Numbers on the top indicate the dilution factor. A set of data from one of the three experiments, which yielded essentially the same results, is shown.