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. 2021 Dec 24;298(2):101382. doi: 10.1016/j.jbc.2021.101382

Figure 2.

Figure 2

Flow cytometry analysis of glycoengineered HEK293 cells with lectins and mAbs. HEK293 cells stably engineered with KO (Δ) and KI (+) as indicated were analyzed with PNA lectin and mAbs 3C9, HH8, and NUH2 at different concentrations (color coded). Radar charts show mean fluorescence intensities (MFIs), and solid/dashed lines represent binding with and without pretreatment with neuraminidase. Charts represent single experiments, and independent experiments were performed at least three times with similar results. The predicted glycan structures produced by the glycoengineered HEK293 cells are shown in first panel (PNA). HEK293, human embryonic kidney 293 cell line; KI, knockin; mAb, monoclonal antibody; PNA, peanut agglutinin.