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. 2022 Jan 12;12:722349. doi: 10.3389/fimmu.2021.722349

Figure 2.

Figure 2

CD209/CD14+ DC are enriched in the joint of PsA and RA patients. (A) Left, representative flow plot of CD209/CD14+ DC frequency [showing the frequency of the parent population (CD11ch/CD14h)] in PBMC, SFMC and synovial tissue (ST). Right, Representative dot plots of frequency of CD209/CD14+ DC in PsA PBMC (n=14), SFMC (n=6), ST (n=10), CD40 expression PBMC (n=5), SFMC (n=3), ST (n=5), CD80 PBMC (n=6), SFMC (n=3), ST (n=7) and RA PBMC (n=21), SFMC (n=12), ST (n=12), CD40 expression PBMC (n=9), SFMC (n=4), ST (n=6), CD80 PBMC (n=12), SFMC (n=8), ST (n=8). Data are represented as mean ± SEM [frequency of the parent population (CD209/CD14+)] and Differences among groups were evaluated by non-parametric One-way ANOVA, Kruskal-Wallis test *p < 0.05. **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Endocytosis was evaluated in parallel at 4 and 37°C following antigen incubation. Left Representative flow plot of Receptor-dependent endocytosis (with OVA-DQ) in PBMC and ST. Right, Trending line of OVA-DQ update (expressed as ration between 37 and 4°C) in paired PBMC and ST (PsA n=6 and RA n=5). (C) SPICE algorithm flow cytometric analysis of PBMC (PsA n=14, RA n=21), SFMC (PsA n=5, RA n=9) and ST (PsA n=10, RA n=10) CD209/CD14+ DC displaying the frequency of the chemokines receptors CCR6 (red arc), CCR7 (yellow arc), CXCR3 (green arc), CXCR4 (pink arc) and CXCR5 (blue arc) and corresponding (D) Dot plot of different chemokine receptors co-expression. Data are represented as mean ± SEM and differences among groups were evaluated by non-parametric One-way ANOVA, Kruskal-Wallis test *p < 0.05. **p < 0.01, ***p < 0.001.