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. 2022 Jan 12;15:757628. doi: 10.3389/fnins.2021.757628

FIGURE 5.

FIGURE 5

Electroacupuncture relieves pain sensation through the rACCGlu-vlPAG circuit, without ameliorating anxiety-like behaviors in SNI mice models. (A) Schematic diagram of the experimental process. (B) Schematic diagram of chemogenetics. (C) Time course of the SNI-induced changes in the PWTs. (D) Time spent in the open arms by the mice in the EPM test. (E) Entries in the open arms by the mice in the EPM test. (F) Representative movement trajectory diagram and activity heatmap of the SNI + mCherry + CNO, SNI + mCherry + CNO + EA, SNI + hM3D + CNO + EA, and SNI + hM3D + CNO groups in the EPM test. (G) Time spent in the center of the field by the mice in the OFT. (H) Center distance covered by the mice in the OFT. (I) Travel distance in the center area in the OFT. (J) Representative movement trajectory diagram and activity heatmap of the SNI + mCherry + CNO, SNI + mCherry + CNO + EA, SNI + hM3D + CNO + EA, and SNI + hM3D + CNO groups in the OFT. Data are presented as means ± standard errors of the means. *P < 0.05 vs. the SNI + mCherry + CNO group; #P < 0.05 vs. the SNI + hM3D + CNO group; *P < 0.05 vs. the SNI + hM3D + CNO + EA group; NS, not significant. n = 10–12 mice per group in (C–E); n = 9–10 mice per group in (G–I).