TABLE 1.
Determination of Borrelia isolates from ticks which fed on mice infected by B. garinii
| Mouse | Infecting serotype | Challenge ticks
|
Xenodiagnostic ticks
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of:
|
No. of isolates ofb:
|
No. of IF-positive ticksc | No. of:
|
No. of isolates of:
|
||||||||
| Isolates | Tubesa | Bg | Ba | Bg + Ba | Isolates | Tubesd | Bge | Ba | Bg + Ba | |||
| 1 | 4 | 4 | 14 | 0 | 4 | 0 | 8 | 2 | 3 | 1 | 1 | 0 |
| 2 | 4 | 1 | 11 | 0 | 1 | 0 | 5 | 5 | 5 | 3 | 0 | 2 |
| 3 | 4 | 3 | 12 | 1f | 2 | 0 | 8 | 1 | 1 | 0 | 1 | 0 |
| 4 | 4 | 5 | 9 | 0 | 5 | 0 | 9 | 2 | 2 | 1 | 1 | 0 |
| 5 | 4 | 8 | 13 | 0 | 8 | 0 | 6 | 4 | 4 | 4 | 0 | 0 |
| 6 | 4 | 1 | 13 | 0 | 0 | 1 | 3 | 5 | 6 | 5 | 0 | 0 |
| 7 | 5 + 6 | 6 | 9 | 6 | 0 | 0 | 0 | 0 | 1 | |||
Each culture tube was inoculated with one challenge tick.
Bg, B. garinii; Ba, B. afzelii.
Ten ticks were tested.
Each culture tube was inoculated with five xenodiagnostic ticks.
Results shown in bold indicate that the isolates reacted with L32 1G3, a monoclonal antibody against B. garinii OspA serotype 4.
Identified by RFLP analysis in one culture tube with nonmotile spirochetes.