Figure 2.

Alanine scanning mutagenesis of the α-helix 3 of FOXO1. (A) The 3D structure of the FOXO1 forkhead domain with the DNA double helix was visualized using PyMOL (PDB ID: 3COA). (B) HEK293T cells were co-transfected with wild-type or mutated pCMV-FOXO1, DBE6x-luciferase reporter and the pEF-β-galactosidase vector as internal control. The luminescence and the β-galactosidase activity were measured after 24 h. Average of 3 independent experiments is shown with standard error of the mean. Statistical analysis was calculated according to bilateral Student t-test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).