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. 2022 Jan 25;12:1309. doi: 10.1038/s41598-022-05334-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of FOXO1 mutants, phosphorylation and interaction with ATG7. (A) HEK293T cells were transfected with wild-type or mutated pCMV-FOXO1 before protein extraction. Total protein lysates were analyzed by western blot with anti-phospho-FOXO1 (phospho-Thr24, phospho-Ser256 and phospho-Ser319), anti-FOXO1 and anti-β-actin antibodies. (B) HEK293T cells were transfected with wild-type or mutated pCMV-FOXO1 before protein extraction. FOXO1 was immunoprecipitated with anti-FOXO1 antibody and analyzed by western blot with anti-FOXO1 and anti-ATG7 antibodies. Original unprocessed images are available in Supplementary Fig. S2. (C) Schematic conclusion of cellular and molecular mechanisms of FOXO1 DBD versus Nt mutations.