Table 1.
Summary of molecular impact of FOXO1 DBD mutations.
| Position | A166V | L168V | L183P | I186T | S205N | N216K |
|---|---|---|---|---|---|---|
| Forkhead domain localization | α-Helix 1 | α-Helix 1 | α-Helix 2 | α-Helix 2 | α-Helix 3 | α-Helix 3 |
| Amino acid conservation among FOXO family members | Yes | Yes | Yes | Yes | Yes | Yes |
| Predicted damage (mutationassessor.org) | Low | High | High | High | Low | High |
| DBE transcriptional activity (significant decreased compared to WT) | * | *** | *** | *** | ns | ** |
| DNA affinity (significant decreased compared to WT) | ns | ns | ** | * | * | ** |
Statistical analysis was calculated according to bilateral Student t-test (ns, non-significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001).