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. 2022 Jan 25;13:493. doi: 10.1038/s41467-022-28040-1

Fig. 3. Ultrasound response is mediated by the entry of extracellular calcium.

Fig. 3

a Calcium responses from a single neuron during ultrasound stimulation before, during and after treatment with the sodium channels blocker tetrodotoxin (TTX). b Quantification of area under the curve before, during and after TTX treatment (n = 3 independent experiments, one-way ANOVA p = 0.0004, Tukey’s post comparison (control vs. TTX)). c Diagram of the Ace2N voltage indicator genetic construct and representative fluorescence image of neurons transfected with this construct. d Voltage responses to ultrasound. (n = 4 independent experiments). e Voltage responses of neurons in calcium-free media (n = 2 independent experiments). Mean trace is solid and SEM is shaded. Bar graph values represent mean ± SEM.