Fig. 3. FNLS-ABE7.10(AW) base editor-based functional screening of m⁶A sites in H1 hESCs.

a Flow chart of ABE-based screening of m⁶A sites is important for endoderm specification. b Representative CXCR4 FACS gating strategy for sorting CXCR4⁻ and CXCR4⁺ populations, respectively. SSC side scatter. c, d Scatter plot showing the strategy to determine the sgRNAs significantly enriched in CXCR4⁻ (c) and CXCR4⁺ (d) populations, respectively. e Heatmap showing the normalized counts of sgRNAs enriched in CXCR4⁻ or CXCR4⁺ populations for each replicate. f, g GO-enrichment analyses of the genes targeted by the sgRNAs significantly enriched in the CXCR4⁻ (f) or CXCR4⁺ (g) populations, respectively.