Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 25;13:478. doi: 10.1038/s41467-022-28106-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a, b siRNA knockdown efficiency of SDHAF1 in hESCs (a) and at day 3 of DE differentiation (b), respectively (n = 6 biologically independent samples), measured by RT-qPCR. si siRNA, NC non-targeting control. P-values were calculated vs. siNC (one-way ANOVA with Tukey’s post hoc test). c, d Representative (c) and quantitative (d) flow cytometry analyses of CXCR4 expression in cultures derived from WT or SDHAF1-mut hESCs that with NC or SDHAF1-targeting siRNAs at day 3 of DE differentiation (n = 6 biologically independent samples). ns non-significant (one-way ANOVA with Tukey’s post hoc test). e, f siRNA knockdown efficiency of ADM in hESCs (e) and at day 3 of DE differentiation (f) respectively (n = 6 biologically independent samples), measured by RT-qPCR. si siRNA, NC non-targeting control. P-values were calculated vs. siNC (one-way ANOVA with Tukey’s post hoc test). g, h Representative (g) and quantitative (h) flow cytometry analyses of CXCR4 expression in cultures derived from WT or ADM-mut hESCs that with NC or ADM-targeting siRNAs at day 3 of DE differentiation (n = 6 biologically independent samples). ns non-significant (one-way ANOVA with Tukey’s post hoc test). i, j mRNA levels of SDHAF1 (i) and ADM (j) in overexpressed hESCs (n = 6 biologically independent samples), measured by RT-qPCR. Dox, doxycycline (one-way ANOVA with Tukey’s post hoc test). k, l Representative (k) and quantitative (l) flow cytometry analyses of CXCR4 expression in cultures derived from WT and overexpressed hESCs at day 3 of DE differentiation (n = 6 biologically independent samples). Dox doxycycline (one-way ANOVA with Tukey’s post hoc test). Data of all relevant panels are presented as means ± SD. Source data are provided as a Source Data file.