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. 2022 Jan 25;12:1291. doi: 10.1038/s41598-022-05457-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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5-mC methylation of AcMNPV transcripts. (a) No significant correlation was observed between the coverage and the number of methylated nucleotides in the raw fraction. Yellow dots indicate positions designated for further analysis. The plot was made with the ggplot2 r package⁵³. (b) Test statistics of UUAC*CG sequence (methylated positions are labelled by asterisks). This panel plots a distribution of test statistics for motif-matching and non-motif-matching sites for all of the provided motives. The plot was made with the ggplot2 r package⁵³. (c) A putative methylated cytosine of the readthrough part of ODV-E25 transcript (5-mC labelled by asterisk). The red curves indicate the electric signals, while the densities indicate the alternative signal levels. The plot was made with the tombo 1.5.1 software⁴⁴.