Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 25;12:1297. doi: 10.1038/s41598-022-05301-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Flow cytometry analysis of NPs uptake under the different conditions assayed. (a,c,e,g) present the fluorescence intensity collected in the green channel under the different conditions assayed: (a) no uptake inhibition; (c) clathrin-dependent endocytosis inhibition (sucrose); (e) caveolae-dependent endocytosis inhibition (genistein); and (g) energy-dependent uptake inhibition (4 °C) respectively, representing the fluorescence intensity due to FITC associated to the polymer in NPs, and thus associated to the presence of NPs. (b,d,f,h) show the corresponding fluorescence intensity obtained in the red channel for the lipophilic fluorophore (NR) and the hydrophilic fluorophore (Rh), associated to the uptake of either lipophilic or hydrophilic molecules encapsulated in the NPs under the different conditions of uptake inhibition assayed. Statistically significant differences in fluorescence intensity (%) values between plain NR-FITC-PLGA NPs and Tf- NR-FITC-PLGA NPs are highlighted as *p < 0.0332; **p < 0.0021; ***p < 0.0002; ****p < 0.0001. Statistically significant differences in fluorescence intensity (%) values between plain Rh-FITC-PLGA NPs and Tf- Rh-FITC-PLGA NPs are highlighted as ^#p < 0.0332; ^##p < 0.0021; ^###p < 0.0002; ^####p < 0.0001.