Table 1.
Summary of the selectivity of allele-specific editing on 18 cancer hotspot mutations using perfect, truncated, and mismatched gRNAs.
| Gene | Protein change | Nucleotide change | Targetability | ||
|---|---|---|---|---|---|
| Perfect gRNA | Truncated gRNA | Mismatched gRNA | |||
| ERBB2 | L755S | c.2264T>C | Yes | Yes | Yes |
| IDH1 | R132H | c.395G>A | Yes | Yes | Yes |
| PIK3CA | H1047R | c.3140A>G | Yes | Yes | Yes |
| VHL | R200W | c.598C>T | Yes | Yes | Yes |
| BRAF | V600E | c.1799T>A | No | Yes | Yes |
| EGFR | L858R | c.2573T>G | No | Yes | Yes |
| EGFR | T790M | c.2369C>T | No | Yes | Yes |
| GNAS | R201C | c.601C>T | No | Yes | Yes |
| KRAS | Q61H | c.183A>C | No | Yes | Yes |
| CTNNB1 | T41A | c.121A>G | No | No | Yes |
| ERBB2 | S310F | c.929C>T | No | No | Yes |
| FBXW7 | R465C | c.1393C>T | No | No | Yes |
| FGFR3 | Y373C | c.1118A>G | No | No | Yes |
| KRAS | G12D | c.35G>A | No | No | Yes |
| NRAS | G12D | c.35G>A | No | No | Yes |
| TP53 | R248W | c.742C>T | No | No | Yes |
| PIK3CA | E542K | c.1624G>A | No | No | No* |
| RAC1 | P29S | c.85C>T | No | No | No* |
*Mutants lacking efficient gRNA for targeting.