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. 2022 Jan 25;12:1340. doi: 10.1038/s41598-022-05060-x

Figure 2.

Figure 2

Establishment of assays for sensitive asprosin detection. (A) Testing of pc-asp sensitivity using surface plasmon resonance (SPR). (left) Sensorgrams showing detection of recombinant asprosin injected at 0–80 nM (0–2960 ng/ml) onto pc-asp immobilized to the sensor chip. (right) Robust and linear response of asprosin injected at 0–5 nM (0–185 ng/ml) onto pc-asp immobilized on chip. (B) Asprosin detection by direct ELISA using pc-asp as detector antibody (1:2000 dilution, 0.5 µg/ml). Robust and linear ELISA signal response of immobilized recombinant asprosin. (C) Sensitive detection of asprosin in solution by sandwich ELISA. Pc-asp was immobilized as capture antibody (3 μg/ml) and mab anti-asprosin (AdipoGen Life Sciences Inc., San Diego, USA) was used as detector antibody (1:2000 dilution, 0.5 µg/ml). Data points represent mean ± SD from duplicates. Data were analyzed using Graphpad Prism version 8.0.2.