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. 2022 Jan 25;12:1340. doi: 10.1038/s41598-022-05060-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Asprosin serum levels increase after 30 min of running exercise. (A) Schematic diagram representing the overall study design showing exercise and rest regimens and blood sampling time points. (B) (Left) Absolute serum asprosin concentration (ng/ml) for each subject before and after running exercise (n = 15 subjects); t0: immediately before running exercise (after 30 min rest in sitting position); t1: immediately after exercise; t2, t3, and t4 represent 30 min, 60 min, and 120 min after exercise, respectively. (right) Relative serum asprosin concentration after running exercise (t1, t2, t3, and t4) normalized to its baseline concentration before exercise (t0) and represented as percentage. A significant increase of asprosin concentration was observed immediately after running exercise; asprosin concentration (c) at t0 = 100%; c(asprosin) t1/c(asprosin) t0 = 123.7 ± 42.3%; c(asprosin) t2/c(asprosin) t0 = 111.3 ± 25.5%; c(asprosin) t3/c(asprosin) t0 = 114 ± 23.40%; c(asprosin) t4/c(asprosin) t0 = 111.5 ± 31.06%; *P = 0.0385. Serum (25 µl) fourfold diluted in PBS (75 µl) for asprosin concentration analysis. Data were analyzed using Graphpad Prism version 8.0.2.