Figure 2.
Overexpression of antiapoptotic Bcl-2 members affected metformin effects on leukemic cells. (A) Mec-1 cell line and stable cell lines overexpressing Bcl-XL or Mcl-1 were incubated with 2 mM metformin for 3 days and plasma membrane expression of the stress ligands MICA/B and ULBP1, the integrin ICAM-1, the death receptors Fas, DR4 and DR5, the (B) cell marker CD20 and MHC class I HLA were analyzed by FACs analysis. Data represent the amount of protein compared to control, non-treated, cells. The bar graphs represent means ± SD of 3 independent experiments; *p < 0.05, **p < 0.01, ***p < 0.005 student t-test compare to control cells.