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. 2022 Jan 25;12:1337. doi: 10.1038/s41598-022-05246-3

Table 3.

Statistical analysis of positivity rates for various bacterial species or groups stratified by preincubation time.

PI N (%) Chi-Sq CATT OR (95% CI) P value OR/h (95% CI)
BHS
 < 5 h 401 (5.41) 0.0807 0.0239 1

1.02 (1.00–1.03)

P = 0.0650

5–10 h 106 (6.87) 1.29 (1.03–1.61) 0.0244
10–20 h 54 (6.62) 1.24 (0.92–1.66) 0.1543
 > 20 h 13 (6.95) 1.30 (0.74–2.31) 0.3616
S. aureus
 < 5 h 1243 (16.79) 0.004 0.006 1

0.99 (0.98–1.00)

P = 0.0468

5–10 h 219 (14.20) 0.82 (0.70–0.96) 0.0126
10–20 h 131 (16.05) 0.95 (0.78–1.15) 0.5937
 > 20 h 17 (9.09) 0.50 (0.30–0.82) 0.0062
E. coli
 < 5 h 1467 (19.81)  < 0.0001  < 0.0001 1

1.04 (1.03–1.04)

P < 0.0001

5–10 h 321 (20.82) 1.06 (0.93–1.22) 0.3701
10–20 h 236 (28.92) 1.65 (1.40–1.94)  < 0.0001
 > 20 h 65 (34.76) 2.15 (1.59–2.93)  < 0.0001
CoNS
 < 5 h 1013 (13.68) 0.0007 0.0007 1

0.97 (0.96–0.99)

P = 0.0001

5–10 h 211 (13.68) 1.00 (0.85–1.17) 0.9986
10–20 h 72 (8.82) 0.61 (0.48–0.79) 0.0001
 > 20 h 19 (10.16) 0.71 (0.44–1.15) 0.1672
Entero-coccus spp
 < 5 h 497 (6.71) 0.4658 0.113 1

0.99 (0.97–1.00)

P = 0.0872

5–10 h 95 (6.16) 0.91 (0.73–1.15) 0.428
10–20 h 45 (5.51) 0.81 (0.59–1.11) 0.1916
 > 20 h 10 (5.35) 0.79 (0.41–1.49 0.4616
K. pneumoniae
 < 5 h 363 (4.90) 0.1493 0.0845 1

1.01 (0.99–1.02)

P = 0.4418

5–10 h 94 (6.10) 1.26 (1.00–1.59) 0.0532
10–20 h 50 (6.13) 1.27 (0.93–1.72) 0.1292
 > 20 h 9 (4.81) 0.98 (0.49–1.93) 0.9551
VGS/ANG
 < 5 h 337 (4.55) 0.1056 0.3110 1

0.98 (0.96–1.01)

P = 0.1461

5–10 h 83 (5.38) 1.19 (0.93–1.53) 0.161
10–20 h 27 (3.31) 0.72 (0.48–1.07) 0.103
 > 20 h 6 (3.21) 0.70 (0.31–1.58) 0.3851
S. pneumoniae
 < 5 h 179 (2.42) 0.1280 0.6289 1

1.00 (0.97–1.02)

P = 0.8620

5–10 h 49 (3.18) 1.32 (0.96–1.83) 0.0859
10–20 h 16 (1.96) 0.81 (0.48–1.35) 0.4166
 > 20 h 2 (1.07) 0.44 (0.11–1.77) 0.2462
Other coliforms
 < 5 h 472 (6.37) 0.0922 0.0190 1

1.02 (1.01–1.03)

P = 0.0058

5–10 h 102 (6.61) 1.04 (0.83–1.30) 0.7266
10–20 h 68 (8.33) 1.34 (1.02–1.74) 0.0327
 > 20 h 17 (9.09) 1.47 (0.88–2.44) 0.1375
NF
 < 5 h 166 (2.24) 0.1450 0.5999 1

0.99 (0.96–1.02)

P = 0.4570

5–10 h 27 (1.75) 0.77 (0.52–1.17) 0.2285
10–20 h 23 (2.82) 1.27 (0.81–1.97) 0.298
 > 20 h 1 (0.53) 0.23 (0.03–1.68) 0.1492

P values for Chi-square and the Cochran-Armitage trend test are shown. Logistical regression analysis was performed both with stratified preincubation times, using the < 5 h preincubation group as reference, and using time as a continuous variable. Statistically significant results are bolded. PI: preincubation, CI: confidence interval, Chi-sq: Chi-square, CATT: Cochran-Armitage Trend Test, OR: odds ratio. N: number of isolates, BHS: beta-hemolytic Streptococcus, CoNS: coagulase-negative Staphylococcus, VGS/ANG: viridans group Streptococcus and S. anginosus group, Mn: mean, NF: non-fermenting gram negative bacilli, TOT: total.