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. 2022 Jan 25;13:501. doi: 10.1038/s41467-022-28158-2

Fig. 5. USP44-TRIM25 increases DSBs by impeding Ku80 recruitment.

Fig. 5

The Vector + sgNC, USP44 + sgNC and USP44 + sgTRIM25 SUNE1 or HONE1 cells were stably constructed. a Representative comet images and quantitative analysis of tail moments for 6Gy-IR-induced DNA damage in the indicated SUNE1 or HONE1 cells, measured by the comet assay. Scale bars, 10 μm. b Representative images and quantitative analysis of the number of γH2AX foci in the indicated SUNE1 and HONE1 cells with or without 6Gy-IR exposure. Scale bars, 10 μm. c The indicated SUNE1 and HONE1 cells were transfected with GFP-Ku80 and then subjected to laser micro-IR and live-cell imaging. Scale bars: 10 μm. d The indicated SUNE1 cells were transfected with EJ5-GFP, infected with or without I-SceI adenovirus and analysed for GFP positivity by flow cytometry. Data in a, b and d are presented as the mean ± SD; the P values were determined using the two-tailed Student’s t-test; n = 20 (a), n = 10 (b), n = 3 (d) repeats from three independent experiments. Source data are provided as a Source Data file.