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. 2022 Jan 25;13:501. doi: 10.1038/s41467-022-28158-2

Fig. 6. Knockdown of TRIM25 reverses the radiosensitizing effect of USP44 in vitro.

Fig. 6

SUNE1 and HONE1 cells were transiently co-transfected with HA-USP44 or the empty vector plasmids plus siTRIM25 or control siRNA. a Clonogenic assays and survival fraction curve analysis (a) and CCK-8 assay (b) of transfected SUNE1 and HONE1 cells after exposure to the indicated IR dose. The absorbance at 450 nm in c is presented as the mean ± SD of n = 4 independent experiments. c, d Cell cycle distribution (c) and apoptosis rate (d) of SUNE1 and HONE1 cells with or without exposure to 6Gy-IR. The cell cycle distribution was detected at 8 h after IR and apoptosis rate was detected at 24 h after IR. Data were presented as the mean ± SD; the P values were determined using the two-tailed Student’s t-test; n = 3 independent experiments. Source data are provided as a Source Data file.