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. 2022 Jan 12;12:803490. doi: 10.3389/fmicb.2021.803490

TABLE 1.

Strains and plasmids used in this study.

Strain/plasmid Description Source or reference
Strains
Escherichia coli
DH5α F φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk,mk+) supE44λ- thi -1 gyrA96 relA1 phoA Weidi Biotechnology Co., Ltd. (Shanghai, China)
DH5αλpir F φ80 lac ZΔM15 Δ(lacZYA-arg F) LAMpir U169 endA1 recA1 hsdR17(rk,mk+) supE44λ- thi -1 gyrA96 relA1 phoA Provided by Prof. Liu Jianzhong of Sun Yat-sen University
BL21 (DE3) F ompT hsdSB(rB mB) gal dcm(DE3) Weidi Biotechnology Co., Ltd. (Shanghai, China)
BW25113 lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 Provided by Prof. Liu Jianzhong of Sun Yat-sen University
BW25113 N05 lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 ΔargB ΔargA ΔptsG::glk ΔgalR::zglf ΔpoxB::acs ΔldhA ΔgltA Provided by Prof. Tao Yong of the Chinese Academy of Sciences
BWD01 BW25113, attPHK::[PT5-dbato]. Optimized gene dbat under the control of PT5 promoter being integrated into the genome of BW25113 This study
BWS01 BW25113, attPHK::[PT5-s189vo]. Optimized mutated gene dbatS189V under the control of PT5 promoter being integrated into the genome of BW25113 This study
N05D01 N05, attPHK::[PT5-dbato]. Optimized gene dbat under the control of PT5 promoter being integrated into the genome of N05 This study
N05S01 N05, attPHK::[PT5-s189vo]. Optimized mutated gene dbatS189V under the control of PT5 promoter being integrated into the genome of N05 This study
Plasmids
pET-32a-DBAT Expression vector, pET-32a with fusion tags and gene dbat under PT7 promoter Laboratory store
pET-32a-Ptac-DBATS189V Expression vector, pET-32a with fusion tags and mutated gene dbatS189V under dual promoter (PT7 and Ptac) This study
pCP20 pSC101 repliconts Flp(λRp) cI857, Cmr, Ampr Provided by Prof. Liu Jianzhong of Sun Yat-sen University
pAH69 Helper plasmid expressing phage HK022 Int, Ampr Provided by Prof. Liu Jianzhong of Sun Yat-sen University
pHKKT5b Integration plasmid, attPHK site PT5 promoter, Kanr Provided by Prof. Liu Jianzhong of Sun Yat-sen University
pHKTT5b-dbato pHKTT5b derivative with the optimized gene dbat under the control of PT5 promoter This study
pHKTT5b-s189vo pHKTT5b derivative with the optimized mutated gene dbatS189V under the control of PT5 promoter This study
HHS Vulnerability Disclosure