Strains
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Escherichia coli
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DH5α |
F– φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk–,mk+) supE44λ- thi -1 gyrA96 relA1 phoA |
Weidi Biotechnology Co., Ltd. (Shanghai, China) |
DH5αλpir |
F– φ80 lac ZΔM15 Δ(lacZYA-arg F) LAMpir U169 endA1 recA1 hsdR17(rk–,mk+) supE44λ- thi -1 gyrA96 relA1 phoA |
Provided by Prof. Liu Jianzhong of Sun Yat-sen University |
BL21 (DE3) |
F–
ompT hsdSB(rB– mB–) gal dcm(DE3) |
Weidi Biotechnology Co., Ltd. (Shanghai, China) |
BW25113 |
lacIq rrnBT14 ΔlacZWJ16
hsdR514 ΔaraBADAH33 ΔrhaBADLD78
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Provided by Prof. Liu Jianzhong of Sun Yat-sen University |
BW25113 N05 |
lacIq rrnBT14 ΔlacZWJ16
hsdR514 ΔaraBADAH33 ΔrhaBADLD78 ΔargB ΔargA ΔptsG::glk ΔgalR::zglf ΔpoxB::acs ΔldhA ΔgltA
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Provided by Prof. Tao Yong of the Chinese Academy of Sciences |
BWD01 |
BW25113, attPHK::[PT5-dbato]. Optimized gene dbat under the control of PT5 promoter being integrated into the genome of BW25113 |
This study |
BWS01 |
BW25113, attPHK::[PT5-s189vo]. Optimized mutated gene dbatS189V under the control of PT5 promoter being integrated into the genome of BW25113 |
This study |
N05D01 |
N05, attPHK::[PT5-dbato]. Optimized gene dbat under the control of PT5 promoter being integrated into the genome of N05 |
This study |
N05S01 |
N05, attPHK::[PT5-s189vo]. Optimized mutated gene dbatS189V under the control of PT5 promoter being integrated into the genome of N05 |
This study |
Plasmids
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pET-32a-DBAT |
Expression vector, pET-32a with fusion tags and gene dbat under PT7 promoter |
Laboratory store |
pET-32a-Ptac-DBATS189V
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Expression vector, pET-32a with fusion tags and mutated gene dbatS189V under dual promoter (PT7 and Ptac) |
This study |
pCP20 |
pSC101 repliconts Flp(λRp) cI857, Cmr, Ampr
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Provided by Prof. Liu Jianzhong of Sun Yat-sen University |
pAH69 |
Helper plasmid expressing phage HK022 Int, Ampr
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Provided by Prof. Liu Jianzhong of Sun Yat-sen University |
pHKKT5b |
Integration plasmid, attPHK site PT5 promoter, Kanr
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Provided by Prof. Liu Jianzhong of Sun Yat-sen University |
pHKTT5b-dbato
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pHKTT5b derivative with the optimized gene dbat under the control of PT5 promoter |
This study |
pHKTT5b-s189vo
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pHKTT5b derivative with the optimized mutated gene dbatS189V under the control of PT5 promoter |
This study |