Figure 1.

(A) MicroCT and skeletal staining reveals an advanced skeletal phenotype in the post-natal day 5 Ramp3 ^-/- mice. Significant increase in the femur BV in Ramp3 ^-/- mice (left) evidently observed in the 3D rendering of the femur bone (middle) (heat map scale: blue = low, red=high). Skeletal staining (right) reveals a remarkable increase in the bone formation in the vertebrae and long-bones in Ramp3 ^-/- mice. (B) MicroCT reveals increase in whole femur and caudal vertebral bone volume in both the 3D bone renderings (top) and quantitative measurements (bottom) at 4 weeks of age. Trabecular analysis at a higher resolution identifies an increase in the trabecular thickness in the tibiae in both, the 3D models (top) and quantifications (bottom) in the Ramp3 ^-/- mice. (Heat map scales in 3D models: Coloured scale- blue=low and red=high; Black and White scale – black = low and white= high).