Figure 4.

(A) Haematoxylin and eosin-stained tibia sections reveal increased trabecular bone (red arrows) in the Ramp3 ^-/- mice at 8 weeks of age. (B) Histological measurements identify increase in the bone surface, osteoblast number and osteoblast covered bone surface (top) in the Ramp3 ^-/- mice. No differences in the osteoclast numbers and osteoclast covered bone surface (bottom) identified. (C) Haematoxylin and eosin-stained tibia sections of the growth plate show increase in the growth plate thickness (blue brace brackets). (D) Quantitative analysis of the growth plate reveals that the proliferative zone, but no the hypertrophic zone contributes to the gross increase in the growth plate thickness.