Figure 3.

E-cadherin antibody, which specifically blocks cellular junctions, and DTT, which inactivates extracellular adhesions through disulfide exchange, were used to perturb cell-cell adhesions while maintaining extracellular calcium to validate our Ca- results. Phase contrast images show morphology for cells treated with control media (A and B), calcium-free media (C and D), antibody (E and F), and DTT (G and H). Scale bar represents 60μm. Morphology quantification (I) reveals that morphological changes induced by both antibody and DTT are consistent with our Ca- results. Cross-category statistics for cells grown on TCP were not included as they were all found to be nonsignificant. For (G), n = 50. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.