Figure 6.

(A) Cell mean velocity was measured by PIV analysis performed on time-lapse images of cell monolayers treated with Ca + or Ca- media. Regions of interest, with a field of view of 672 μm × 672 μm, were registered and imaged for 48 h post-Ca+/- treatment at an interval of ∼4 min. Both softening of the substrate modulus and disruption of cell-cell contacts were shown to increase cell motility to different degrees. (B–E) Representative heatmaps for each condition illustrate this trend. Additionally, samples with lower mean migration showed greater homogeneity in local velocity magnitudes. Scale bar represents 100 μm. For (A), n = 32. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001.