Figure 5.

F1459 (in the 15th position on S6) is responsible for gating the DII-III fenestration of hNav1.5. Chi1(χ₁) dihedral angle of F1459 (purple) plotted alongside bottleneck radius (red) over time with a moving average window of 20 ns (A); bottleneck radius distributions resultant from individual sidechain deletions of bottleneck residues (B) showed that deletion of the F₁₅ aromatic sidechain had a profound effect on creating a consistently wide bottleneck radius; representative snapshots for F1459 being in the “down” configuration resulting in a wide fenestration bottleneck radius (C); compared with F1459 being in the “up” configuration, resulting in a narrowed fenestration bottleneck radius (D).