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. 2022 Jan 12;9:775599. doi: 10.3389/fcell.2021.775599

FIGURE 5.

FIGURE 5

Central memory cells (Tcm) expressing hHRS3-CAR were enriched in mice and conferred long-lasting immunity to HL. (A) Survival of mice is shown as a Kaplan-Meier curve (data shown combined from two independent experiments, n = 12/group). Survival was not statistically different for NC mice versus CD19; p = 0.8037. p < 0.0001 for the comparison of HRS3 group versus CD19 mice. p < 0.0001 for the comparison of hHRS3 group versus CD19 mice. NC, non-transduced T cells. (B) Flow cytometry detection of tumor cell involvement in the liver of mice in each group. The control group was CD19 group (n = 3), and the experimental group was HRS3 and hHRS3 groups (n = 4). (C) Spleen, (D) blood, and (E) bone marrow from surviving CD30-CAR-treated mice (n = 3) were analysed for the number of CAR-T cell subsets using flow cytometry (mean ± SD). T cells are functionally divided into four subsets: naïve (CCR7+CD45RO−); central memory (CCR7+CD45RO+); effector memory (CCR7−CD45RO+); and terminal effector (CCR7−CD45RO−), according to the cell surface expression of CCR7 and CD45RO. Each experiment included 6 mice per group and was repeated twice (total n = 12 mice per group). (B) data shown are representative or (C–E) combined from two independent experiments (n = 12). * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001 vs control (CD19).