Supplementary Figure 7.

Overall richness and diversity as well as composition of the microbiota in different gut segments from mice that received iron-deficient 45%HFD and regular-iron 45% HFD. A. Chao1 index. B. Shannon index. Data are presented as means ± S.E.M. Student's t test were used for significance assessments. DM = duodenum mucosa, DC = duodenum chyme, IM = ileum mucosa, IC = ileum chyme, CE = cecum chyme. C. Weighted UniFrac principal coordinates analysis. Each dot represents a gut microbiota community of iron-deficient or regular-iron group. Each principal coordinate (PC) explains certain percentage of variation as shown in parentheses. DEF = iron-deficient group, REG = regular-iron group.