Overall richness and diversity as well as composition of the microbiota in different gut segments from mice that underwent VSG and sham surgery. A. Chao1 index. B. Shannon index. Data are presented as means ± S.E.M. Student's t test were used for significance assessments. C. Weighted UniFrac principal coordinates analysis. Each dot represents a gut microbiota community of VSG or SHAM group. Each principal coordinate (PC) explains certain percentage of variation as shown in parentheses. Samples were collected from mice of the study related to Figure 1. VSG = vertical sleeve gastrectomy, SHAM = sham surgery, DM = duodenum mucosa, DC = duodenum chyme, IM = ileum mucosa, IC = ileum chyme, CE = cecum chyme.