Figure 5.

Mitochondrial unfolded protein response (UPR^mt) subsequent to 7 days of muscle disuse.A–C, representative Western blots and corresponding graphs for ATF4, CHOP, ATF5, CPN10, GRP75, LonP, and ClpP from whole-muscle protein samples from 7-day DEN EDL muscle. D, heat map of DESeq RNA-Seq data showing differential expression of select genes in the context of the UPR^mt. The data are expressed as log2 fold change following 7 days of denervation as compared with control (reduced gene expression, red; enhanced gene abundance, blue; no change in gene expression, white; FDR < 0.05 and p < 0.05, see Table S3 for expanded data table including both DESeq and EdgeR analysis). Bars depict means ± SE; n = 4 to 10 (A–C); Δp < 0.05, main effect of denervation; δp < 0.05, main effect of genotype; ∗p < 0.05, DEN versus CON of same genotype by repeated-measures two-way ANOVA. ATF4, activating transcription factor 4; ATF5, activating transcription factor 5; CHOP, C/EBP homologous protein; ClpP, caseinolytic mitochondrial matrix peptidase proteolytic subunit; CON, control; CPN10, 10 kDa mitochondrial chaperone; DEN, denervated; EDL, extensor digitorum longus; FDR, false discovery rate; GRP75, glucose-regulated protein 75; LonP, lon protease homolog, mitochondrial.