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. 2022 Jan 12;9:810118. doi: 10.3389/fcell.2021.810118

FIGURE 2.

FIGURE 2

Impaired ability to bind to the oolemma in Izumo1 KO rat sperm. (A) The eggs recovered from the ampulla of the superovulated females mated with Izumo1 Het or Izumo1 KO male. The nuclei were stained by Hoechst 33342. Scale bar = 100 µm. (B) The average ratio of the ZP-penetrated eggs (left) or two-pronuclear zygotes (right) to the total recovered eggs. ns represents no statistical difference. ***p < 0.001. (C) The sperm-egg fusion was assessed by staining the nuclei with Hoechst 33342. Fused sperm are indicated by the fluorescence signals of the swollen sperm nuclei. Scale bar = 50 µm. (D) The average numbers of the fused spermatozoa (left) and oolemma-bound spermatozoa (right). Izumo1 WT and Het rat sperm were used as control. **p < 0.01 (E) The sperm-egg adhesion was captured at 0, 0.5, 2, and 5 h after insemination. Scale bar = 50 µm.