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. 2022 Jan 12;9:810118. doi: 10.3389/fcell.2021.810118

FIGURE 3.

FIGURE 3

Reduced binding of the acrosome-reacted Izumo1 KO mouse sperm to the oolemma. (A) A representative image of mouse sperm adhered to egg membrane. Spermatozoa with the EGFP signals are acrosome-intact, whereas the ones without EGFP are acrosome-reacted (yellow arrowhead). DsRed2 is expressed in the sperm midpiece. The sperm nuclei were stained by Hoechst 33342. Scale bar = 50 µm (B) The total numbers of oolemma-bound spermatozoa. ns represents no statistical difference. (C) The ratios of the acrosome-reacted sperm to the total number of oolemma-bound sperm. *p < 0.05.