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. 2022 Jan 12;9:779169. doi: 10.3389/fcell.2021.779169

FIGURE 4.

FIGURE 4

Knocking down HIF-2α suppresses aerobic glycolysis and invasion of breast cancer cells. (A) HIF-2α and glycolytic-related proteins in parental and HIF-2α-knockdown MDA-MB-231 cells were determined by Western blotting analysis. β-tubulin was included as an internal control. Production of lactate (B) and glucose consumption (C) in parental and HIF-2α knockdown MDB-MB-231 cells. (D) HIF-2α and glycolysis-related protein levels were determined by Western blotting in parental and HIF-2α knockdown BT549 cells. β-tubulin was included as an internal control. Production of lactate (E) and glucose consumption (F) in parental and HIF-2α knockdown BT549 cells. (G–H) Invasion of MDA-MB-231 (G) and BT549 (H) and their derivatives treated with or without 20 mM oxamate was analyzed by a transwell assay sistern. Data are shown as mean ± s.d of triplicate measurements (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. (Student’s t-test).