FIGURE 5.

SIPA1/HIF-2α axis blockade suppresses aerobic glycolysis and breast cancer growth and metastasis in a xenograft mouse model. (A) The schematic overview of the xenograft experimental workflow. Oxamate treatment on 231si cell xenografted tumor formation experiment was done twice (1# and 2#). (B) Tumor volumes in the four groups of mice were measured from day 5 to day 19. (C) The tumors in each group were photographed and weighed. (D) Lactate levels in tumor tissues were determined in each group, n = 3. (E) mRNA levels of glycolytic rate-limiting genes in tumor tissues of mice (n = 3 in each group) were determined by qRT-PCR. Data are shown as mean ± s.d. The experiments were done in triplicate. (F) Immunohistochemical analyses of HIF-2α, SLC2A1 and PDK1 in tumor tissues derived from two groups of mice were performed. (G) Lungs from four groups of mice were photographed (left panel) and the number of nodules observed on the lung surface were counted (right panel). (H) Representative histopathological images of HE staining in the lungs were presented. Scale bar: 1 mm. Data are mean ± s.d. of triplicate measurements. *p < 0.05, **p < 0.01, ***p < 0.001. (Student^’s t-test).