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. 2022 Jan 12;8:815947. doi: 10.3389/fnut.2021.815947

Figure 4.

Figure 4

LBE extends lifespan depending on sir-2.1 under unfavorable conditions. (A) Survival curves of N2 worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 80, mean lifespan 11.6 days; 5 mg/ml LBE: n = 82, mean lifespan 15.5 days; p < 0.0001). (B) Survival curves of hsf-1 (sy4421) worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 72, mean lifespan 9.7 days; 5 mg/ml LBE: n = 97, mean lifespan 11.1 days; p < 0.0001). (C) Survival curves of aak-2 (ok524) worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 100, mean lifespan 13.4 days; 5 mg/ml LBE: n = 114, mean lifespan 17.1 days; p < 0.0001). (D) Survival curves of daf-16 (mu86) worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 86, mean lifespan 10.8 days; 5 mg/ml LBE: n = 90, mean lifespan 16.6 days; p < 0.0001). (E) Survival curves of xbp-1 (zc12) worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 61, mean lifespan 12.7 days; 5 mg/ml LBE: n = 80, mean lifespan 17.3 days; p < 0.0001). (F) Survival curves of sir-2.1 (ok434) worms cultured with vehicle or 5 mg/ml LBE at 20°C (control: n = 119, mean lifespan 10.8 days; 5 mg/ml LBE: n = 63, mean lifespan 10.6 days; p = 0.981). All the results are representative of at least two independent lifespan experiments. Log-rank test was used to assess significance (see also Supplementary Table 4). LBE, total water extracts of L. barbarum berry.