Fig. 5.

Bumpel and Kumpel are both expressed in CNS glial cells. (A,B) Schematic representation of the genomic loci of bumpel (CG6723) and kumpel (CG42235). Transcription is from left to right, coding exons are colored, five different isoforms are generated from the kumpel gene. The position of the stop codon mutations and the endogenously integrated V5 tags are indicated. GFSTF indicates the position of a MiMIC insertion. (C–H) Confocal analysis of third instar larval brains and adult brains stained for Rumpel, Bumpel^V5 and Kumpel^PC::V5 as indicated. Red dashed lines indicate the position of orthogonal planes shown in C′,E′,G′. (C,C′) Rumpel localizes predominantly in the ensheathing glial cells (arrowhead). (D) Maximum projections and (D′) single focal plane showing Rumpel localization in the adult brain. Rumpel is enriched in ensheathing glia (arrowheads). (E,E′) Bumpel^V5 localizes to ensheathing glia (arrowhead) and cortex glial cells (arrows). Additional expression is noted in the neuropil (asterisk). (F,F′) In the adult nervous system, Bumpel localizes as detected for Rumpel. In addition, Bumpel^V5 is found in the developing eyes (asterisk). (G,G′) Kumpel^PC::V5 localizes predominantly to cortex glial cells (arrows). No Kumpel^PC::V5 can be detected in the neuropil (asterisk). (H,H′) Kumpel^PC::V5 localizes to the cortex glial cells in the adult brain (arrows). Only weak expression in adult ensheathing glia is noted (arrowhead, H). No Kumpel^PC::V5 can be detected in the neuropil (asterisk).