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. 2022 Jan 12;9:809942. doi: 10.3389/fcell.2021.809942

FIGURE 2.

FIGURE 2

Overexpression of HDAC6 increases poly (GA) levels. (A,B) Immunoblots (A) and densitometric analysis of immunoblots (B) for the indicated proteins to examine the levels of acetylated tubulin (Ac-Tub) in HEK293T cells co-expressing 66R with vector, HDAC6WT or HDAC6Mut (n = 3 independent experiments). Data are presented as mean ± SEM. In (B), (left) n.s. not significant, two-tailed unpaired t test (right) **p < 0.01, ****p < 0.0001, one-way ANOVA, Tukey’s multiple-comparison test. (C) qRT-PCR was performed to assess 66R mRNA levels in HEK293T cells co-expressing 66R with vector, HDAC6WT or HDAC6Mut (n = 5 independent experiments). Data are presented as mean ± SEM, n.s. not significant, one-way ANOVA, Tukey’s multiple-comparison test. (D) Poly (GA) and poly (GP) levels in HEK293T cell lysates were measured by immunoassay (n = 5 independent experiments). Data are presented as mean ± SEM, n.s. not significant; *p < 0.05, **p < 0.01, ****p < 0.0001, one-way ANOVA, Tukey’s multiple-comparison test.