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. 2022 Jan 12;12:809896. doi: 10.3389/fimmu.2021.809896

Figure 6.

Figure 6

ARNO knock-down remodels the type of IL-1β-mediated inflammatory response. (A) mRNA expression as detected by RNA-Seq from Figure 5 for ARNO (Cyth2), IL-6, CXCL9, CCL9, CLDN1, MMP3, MMP13, TNFRSF11b and TNFSF11. Differentially expressed genes with a adjp < 0.01 are shown by *. (B–D) RNA was isolated from unstimulated and IL-1β-stimulated naïve SFs upon Allstars/ARNO siRNA transfection. Relative mRNA expression of genes shown in (A) was evaluated by RT-qPCR using the ΔΔCt method and actin as housekeeping gene. (B) CLDN1, Cxcl9, MMP13, MMP3, (C) IL-6, Ccl9, and (D) TNFRSF11b and TNFSF11 mRNA relative expression. For (B–D), each dot represents one independent experiment (analysed in triplicate), error bars represent SEM (n≥5), *p < 0.05, ***p < 0.001 versus respective siRNA control, #p < 0.05, ###p < 0.001 versus unstimulated control, statistical significance was evaluated by the Mann-Whitney test.