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. 2022 Jan 12;12:809896. doi: 10.3389/fimmu.2021.809896

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Copyright © 2022 Wang, Çil, Harnett and Pineda

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ARNO knock-down decreases IL-1β-dependent responses of CIA SFs. SFs were expanded from mice undergoing Collagen-Induced Arthritis, and RNA was isolated from unstimulated and IL-1β-stimulated cells upon ARNO knock-down by siRNA or Allstar treatment. ARNO (A) TNFRSF11b and TNFSF11 (B), MMP3, MMP13 (C) and IL-6, CCL2 (D) relative mRNA expression was evaluated by RT-qPCR using the ΔΔC_t method and actin as housekeeping gene. Each dot represents one independent experiment (analysed in triplicate), error bars represent SEM, *p < 0.05, **p < 0.01 versus respective siRNA control, ^#p < 0.05 versus unstimulated control, statistical significance was evaluated by the Mann-Whitney test.