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. 2022 Jan 5;2(1):223–233. doi: 10.1021/jacsau.1c00504

Figure 5.

Figure 5

dTat-Sar-EED4 induces the formation of plasma membrane protrusions and a large intracellular vesicle in plant cells. (A,B) Formation of plasma membrane protrusions (A) and a large intracellular vesicle (B) observed in TEM images of high-pressure frozen A. thaliana root epidermal cells after treatment with 30 μM dTat-Sar-EED4 for 15 min. Yellow arrowheads in (A) indicate plasma membrane protrusions, while those in (B) represent intracellular vesicles (>200 nm in diameter). (C) Formation of membrane invaginations detected in TEM images of high-pressure frozen root epidermal cells after treatment with 30 μM dTat-Sar-EED4 or water for 15 min. Yellow arrowheads indicate the membrane invagination. CW, cell wall; PM, plasma membrane; Cy, cytoplasm.