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. 2022 Jan 26;41:37. doi: 10.1186/s13046-021-02206-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Evaluation of myofibroblastic differentiation of hEML-CM cells. a Upregulation of MYH4 mRNA in A549 and H1299 cells cultured in hEML-CM compared to those cultured in T-CM. Data represent the mean ± SEM from three separate experiments. b Immunofluorescence detection of MYH4 in A549 and H1299 cells cultured in T-CM and hEML-CM. In hEML-CM, the A549 cells showed highly positive staining, while the staining in H1299 cells was less intense. No staining was detected in cells cultured in T-CM. Nuclei were counterstained with DAPI. c Multidimensional Scaling plot of distances between gene expression profiles for A549_hEML-CM, A549_Ctrl, H1299_hEML-CM, H1299_Ctrl, HBEC6-KT, HPAEC, Myoblast and HLF samples. Three samples of each group have been included in the analysis. d Simplified distance matrix heat map showing the mean of the three replicates for each group. *p < 0.05