Fig. 3.

False-color image overlays of two-color multiplex gels from reverse phase fraction 2 prior to spot-matching alignment. Three subunit chains of Hpt are outlined in ovals in the upper right image. The AD pools are represented by green in the upper three images and the controls were labeled red. In the lower images the labeling was reversed to control for potential bias induced by the dyes (AD labeled Green and controls labeled Red). Yellow spots represent approximately equal contribution of intensity from AD and control proteins