a, Schematic representation of large tumour B16-F10 bearing C57BL/6 J mice upon systemic T cell transfer. b, Tumour-growth curves following inoculation of B16F10 following transfer of engineered murine T cells on day 0 and heat treatments on days 1, 3, and 5 (*P = 0.0489 between untreated and cohorts which received cells only on day 8. *P = 0.0295 between cohorts receiving cells and heat versus cells only on day 12, **P = 0.0043 between cohorts receiving cells and heat versus cells only on day 14, two-way ANOVA and Tukey post-test and correction, mean ± SEM is depicted, n = 6–7 biologically independent mice).